Plasmid
CosmoStrep

Part:BBa_K4590012

Designed by: Beatriz Lauriano de Oliveira   Group: iGEM23_USP-Brazil   (2023-10-02)


Malonate incorporation vector

The Streptomyces conjugative vector pIJ6021. Its contains both St(r)epF (BBa_K4590008) and St(r)epG (BBa_K4590009) together.

St(r)epF (BBa_K4590008) is a gene from Streptomyces hygroscopicus that encodes a protein similar to a di-tricarboxylate transporter in Rhizobium, and it is involved in malonate incorporation. St(r)epG (BBa_K4590009) is a gene from Rhizobium leguminosarum that encodes Malonyl-CoA Synthase and is involved in fatty acid synthesis and elongation.

Malonate serves as a precursor in the fatty acid pathway, which is necessary for anthracycline synthesis. Our goal is to increase Cosmomycin production by facilitating the incorporation of malonate into the microorganism and enhancing the flow of fatty acid synthesis (LIMA LOBATO, 2010).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2374
    Illegal EcoRI site found at 2824
    Illegal XbaI site found at 2797
    Illegal PstI site found at 2032
    Illegal PstI site found at 2785
    Illegal PstI site found at 7628
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2374
    Illegal EcoRI site found at 2824
    Illegal PstI site found at 2032
    Illegal PstI site found at 2785
    Illegal PstI site found at 7628
    Illegal NotI site found at 1072
    Illegal NotI site found at 1213
    Illegal NotI site found at 9508
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2374
    Illegal EcoRI site found at 2824
    Illegal BglII site found at 2833
    Illegal BglII site found at 5065
    Illegal BglII site found at 5536
    Illegal BglII site found at 6354
    Illegal BamHI site found at 461
    Illegal BamHI site found at 989
    Illegal BamHI site found at 2803
    Illegal XhoI site found at 1592
    Illegal XhoI site found at 2152
    Illegal XhoI site found at 8425
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2374
    Illegal EcoRI site found at 2824
    Illegal XbaI site found at 2797
    Illegal PstI site found at 2032
    Illegal PstI site found at 2785
    Illegal PstI site found at 7628
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2374
    Illegal EcoRI site found at 2824
    Illegal XbaI site found at 2797
    Illegal PstI site found at 2032
    Illegal PstI site found at 2785
    Illegal PstI site found at 7628
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 439
    Illegal NgoMIV site found at 1692
    Illegal NgoMIV site found at 1846
    Illegal NgoMIV site found at 1924
    Illegal NgoMIV site found at 3353
    Illegal NgoMIV site found at 3479
    Illegal AgeI site found at 2137
    Illegal AgeI site found at 4038
    Illegal AgeI site found at 5308
    Illegal AgeI site found at 5648
    Illegal AgeI site found at 7318
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8848
    Illegal BsaI site found at 9123
    Illegal BsaI site found at 10305
    Illegal SapI site found at 291
    Illegal SapI site found at 525
    Illegal SapI site found at 774
    Illegal SapI site found at 3381
    Illegal SapI.rc site found at 3676


[edit]
Categories
Parameters
None